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ProductsBack/TNNI3K antibody [N2C1], Internal
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TNNI3K(GTX108944) antibody at 1:200 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TNNI3K(GTX108944) antibody at 1:200 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TNNI3K(GTX108944) antibody at 1:200 dilution.

TNNI3K antibody [N2C1], Internal

GTX108944
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetTNNI3K
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Overview

  • Supplier
    GeneTex
  • Product Name
    TNNI3K antibody [N2C1], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51086
  • Target name
    TNNI3K
  • Target description
    TNNI3 interacting kinase
  • Target synonyms
    CARK, CCDD, serine/threonine-protein kinase TNNI3K, cardiac ankyrin repeat kinase, cardiac troponin I-interacting kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ59H18
  • Protein Name
    Serine/threonine-protein kinase TNNI3K
  • Scientific Description
    May play a role in cardiac physiology.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNNI3K using anti-TNNI3K antibody (A06499-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNNI3K antigen affinity purified polyclonal antibody (Catalog # A06499-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNNI3K at approximately 110 kDa. The expected band size for TNNI3K is at 93 kDa.
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Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TNNI3K Polyclonal Antibody, Unconjugated (bs-9458R) at 1:200 overnight at 4°C, DAB staining.
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TargetTNNI3K
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