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Western Blot analysis of AD293, 4T1 cells using Troponin T-C Polyclonal Antibody.
Western Blot analysis of AD293, 4T1 cells using Troponin T-C Polyclonal Antibody.
Western Blot analysis of AD293, 4T1 cells using Troponin T-C Polyclonal Antibody.

TNNT2 Antibody

CSB-PA005579
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTNNT2
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Overview

  • Supplier
    Cusabio
  • Product Name
    TNNT2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7139
  • Target name
    TNNT2
  • Target description
    troponin T2, cardiac type
  • Target synonyms
    cardiomyopathy, dilated 1D (autosomal dominant); cardiomyopathy, hypertrophic 2; CMD1D; CMH2; CMPD2; cTnT; LVNC6; RCM3; TnTC; troponin T type 2 (cardiac); troponin T, cardiac muscle; truncated cardiac troponin T
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP45379
  • Protein Name
    Troponin T, cardiac muscle
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Rat H9C2 cells probed with Rabbit Anti-Troponin T Polyclonal Antibody, Unconjugated (bs-10648R) (green) at 1:50 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue), secondary only(light blue), and isotype control(orange).
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Figure 1. Western blot analysis of Cardiac Troponin T using anti-Cardiac Troponin T antibody (A01154-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac Troponin T antigen affinity purified polyclonal antibody (Catalog # A01154-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cardiac Troponin T at approximately 43KD. The expected band size for Cardiac Troponin T is at 36KD.
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