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TNNT2 / CTNT Antibody (aa275-287)

LS-C186455
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetTNNT2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TNNT2 / CTNT Antibody (aa275-287)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 40kD band observed in Human, fetal Mouse and Rat Heart lysates (calculated MW of 35.6kD according to Human NP_000355.2 and 35.7kD according to Rat NP_036808.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Luedde et al, Cardiovasc Res. 2010 Jun 1;86(3):452-60. PMID:20083571). Recommended concentration: 0.03-0.1 ug/ml.. Peptide-ELISA (1:128000), WB (0.03 - 0.1 µg/ml) Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 40kD band observed in Human, fetal Mouse and Rat Heart lysates (calculated MW of 35.6kD according to Human NP_000355.2 and 35.7kD according to Rat NP_036808.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Luedde et al, Cardiovasc Res. 2010 Jun 1;86(3):452-60. PMID:20083571). Recommended concentration: 0.03-0.1 ug/ml.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7139
  • Target name
    TNNT2
  • Target description
    troponin T2, cardiac type
  • Target synonyms
    cardiomyopathy, dilated 1D (autosomal dominant); cardiomyopathy, hypertrophic 2; CMD1D; CMH2; CMPD2; cTnT; LVNC6; RCM3; TnTC; troponin T type 2 (cardiac); troponin T, cardiac muscle; truncated cardiac troponin T
  • Host
    Goat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cardiac Troponin T using anti-Cardiac Troponin T antibody (A01154-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac Troponin T antigen affinity purified polyclonal antibody (Catalog # A01154-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cardiac Troponin T at approximately 43KD. The expected band size for Cardiac Troponin T is at 36KD.
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