Bio-Connect

Goat anti-TNNT3 Antibody

ORB106868
Biorbyt
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTNNT3
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Biorbyt
  • Product Name
    TNNT3 antibody
  • Delivery Days Customer
    16
  • Application Supplier Note
    WB: Approx 35kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 30.6kDa according to NP_006748.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Marian et al, Circ Res. 1997 Jul;81(1):76-85. PMID: 9201030.).Recommended concentration: 0.01-0.03 microg/ml.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA: 1:128000, WB: 0.01-0.03 μg/ml, IHC-P: 4-8µg/ml ELISA, IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7140
  • Target name
    TNNT3
  • Target description
    troponin T3, fast skeletal type
  • Target synonyms
    beta-TnTF; DA2B2; TNTF; troponin T type 3 (skeletal, fast); troponin T, fast skeletal muscle
  • Host
    Goat
  • Scientific Description
    Goat polyclonal antibody to TNNT3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Everest Biotech
ApplicationsWestern Blot, ELISA
TargetTNNT3
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetTNNT3
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot
TargetTNNT3
  • SizePrice
Anti-TNNT3 Antibody (Biotin)
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot, ELISA
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot
TargetTNNT3
  • SizePrice
Immunohistochemistry analysis in human skeletal muscle and heart muscle tissues using HPA037810 antibody. Corresponding TNNT3 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetTNNT3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
TNNT3 AntibodyLS-C750293
ApplicationsWestern Blot
TargetTNNT3
  • SizePrice
Figure 1. Western blot analysis of TNNT3 using anti-TNNT3 antibody (A05885-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat skeletal muscle issue lysates, Lane 2: mouse skeletal muscle issue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNNT3 antigen affinity purified polyclonal antibody (Catalog # A05885-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNNT3 at approximately 37KD. The expected band size for TNNT3 is at 32KD.
Product group Antibodies
Boster Bio
Anti-TNNT3 Antibody Picoband(r)A05885-2-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetTNNT3
  • SizePrice