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Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with TNR antibody [C1C2], Internal (GTX118246) diluted at 1:500.
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with TNR antibody [C1C2], Internal (GTX118246) diluted at 1:500.
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with TNR antibody [C1C2], Internal (GTX118246) diluted at 1:500.

TNR antibody [C1C2], Internal

GTX118246
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTNR
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Overview

  • Supplier
    GeneTex
  • Product Name
    TNR antibody [C1C2], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.64 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7143
  • Target name
    TNR
  • Target description
    tenascin R
  • Target synonyms
    NEDSTO, TN-R, tenascin-R, janusin, restrictin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92752
  • Protein Name
    Tenascin-R
  • Scientific Description
    Tenascin-R (TNR) is an extracellular matix protein expressed primarily in the central nervous system. It is a member of the tenascin (TN) gene family, which includes at least 3 genes in mammals: TNC (or hexabrachion; MIM 187380), TNX (TNXB; MIM 600985), and TNR (Erickson, 1993 [PubMed 7694605]). The genes are expressed in distinct tissues at different times during embryonic development and are present in adult tissues.[supplied by OMIM]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Tenascin-R/TNR using anti-Tenascin-R/TNR antibody (A04810-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tenascin-R/TNR antigen affinity purified polyclonal antibody (Catalog # A04810-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tenascin-R/TNR at approximately 180 kDa. The expected band size for Tenascin-R/TNR is at 150 kDa.
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