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TOE1 antibody

GTX117545
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTOE1
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Overview

  • Supplier
    GeneTex
  • Product Name
    TOE1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID114034
  • Target name
    TOE1
  • Target description
    target of EGR1, exonuclease
  • Target synonyms
    PCH7, TOE-1, hCaf1z, target of EGR1 protein 1, target of EGR1, member 1 (nuclear)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96GM8
  • Protein Name
    Target of EGR1 protein 1
  • Scientific Description
    Inhibits cell growth rate and cell cycle. Induces CDKN1A expression as well as TGF-beta expression. Mediates the inhibitory growth effect of EGR1.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Sundararaman B, Zhan L, Blue SM, et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell. 2016,61(6):903-13. doi: 10.1016/j.molcel.2016.02.012
    Read this paper
  • Sperandio S, Barat C, Cabrita MA, et al. TOE1 is an inhibitor of HIV-1 replication with cell-penetrating capability. Proc Natl Acad Sci U S A. 2015,112(26):E3392-401. doi: 10.1073/pnas.1500857112
    Read this paper

Datasheet

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Figure 1. Western blot analysis of TOE1 using anti-TOE1 antibody (A09852-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOE1 antigen affinity purified polyclonal antibody (Catalog # A09852-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOE1 at approximately 57 kDa. The expected band size for TOE1 is at 57 kDa.
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