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Toll-Like Receptor 2 Antibody (YA4549, Mouse)

HY-P84852
MedChem Express
TargetTlr2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Toll-Like Receptor 2 Antibody (YA4549, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID24088
  • Target name
    Tlr2
  • Target description
    toll-like receptor 2
  • Target synonyms
    Ly105, toll-like receptor 2
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Toll-Like Receptor 2 Antibody (YA4549) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Toll-Like Receptor 2.
  • UNSPSC
    41116161
  • Species
    Mouse

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Figure 1. Western blot analysis of Tlr2 using anti-Tlr2 antibody (A00131-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tlr2 antigen affinity purified polyclonal antibody (Catalog # A00131-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tlr2 at approximately 89 kDa. The expected band size for Tlr2 is at 89 kDa.
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