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WB analysis of human brain (cerebellum) lysate using GTX88806 TOM1L1 antibody, Internal. Dilution : 0.3microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human brain (cerebellum) lysate using GTX88806 TOM1L1 antibody, Internal. Dilution : 0.3microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of human brain (cerebellum) lysate using GTX88806 TOM1L1 antibody, Internal. Dilution : 0.3microg/ml Loading : 35microg protein in RIPA buffer

TOM1L1 antibody, Internal

GTX88806
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetTOM1L1
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Overview

  • Supplier
    GeneTex
  • Product Name
    TOM1L1 antibody, Internal
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 0.3-1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10040
  • Target name
    TOM1L1
  • Target description
    target of myb1 like 1 membrane trafficking protein
  • Target synonyms
    SRCASM, TOM1-like protein 1, src-activating and signaling molecule protein, target of Myb-like protein 1
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDO75674
  • Protein Name
    TOM1-like protein 1
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of TOM1L1 using anti-TOM1L1 antibody (A07598-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOM1L1 antigen affinity purified polyclonal antibody (Catalog # A07598-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOM1L1 at approximately 55 kDa. The expected band size for TOM1L1 is at 53 kDa.
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