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Western Blot analysis of HuvEc cells using Topo IIIbeta-1 Polyclonal Antibody
Western Blot analysis of HuvEc cells using Topo IIIbeta-1 Polyclonal Antibody
Western Blot analysis of HuvEc cells using Topo IIIbeta-1 Polyclonal Antibody

TOP3B Antibody

CSB-PA004316
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTOP3B
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Overview

  • Supplier
    Cusabio
  • Product Name
    TOP3B Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8940
  • Target name
    TOP3B
  • Target description
    DNA topoisomerase III beta
  • Target synonyms
    TOP3B1, DNA topoisomerase 3-beta-1, DNA topoisomerase III beta-1, topoisomerase (DNA) III beta, topoisomerase III beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95985
  • Protein Name
    DNA topoisomerase 3-beta-1
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TOP3B using anti-TOP3B antibody (A10446-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOP3B antigen affinity purified polyclonal antibody (Catalog # A10446-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOP3B at approximately 90-97 kDa. The expected band size for TOP3B is at 97 kDa.
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