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Western Blot Positive WB detected in: K562 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate All lanes: TOX2 antibody at 4.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 55, 50, 54 kDa Observed band size: 52, 50 kDa
Western Blot Positive WB detected in: K562 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate All lanes: TOX2 antibody at 4.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 55, 50, 54 kDa Observed band size: 52, 50 kDa
Western Blot Positive WB detected in: K562 whole cell lysate, Raji whole cell lysate, Jurkat whole cell lysate All lanes: TOX2 antibody at 4.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52, 55, 50, 54 kDa Observed band size: 52, 50 kDa

TOX2 Antibody

CSB-PA024074LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetTOX2
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Overview

  • Supplier
    Cusabio
  • Product Name
    TOX2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84969
  • Target name
    TOX2
  • Target description
    TOX high mobility group box family member 2
  • Target synonyms
    C20orf100, GCX-1, GCX1, dJ1108D11.2, dJ495O3.1, TOX high mobility group box family member 2, granulosa cell HMG box 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96NM4
  • Protein Name
    TOX high mobility group box family member 2
  • Scientific Description
    Putative transcriptional activator involved in the hypothalamo-pituitary-gonadal system.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TOX2 using anti-TOX2 antibody (A12956-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOX2 antigen affinity purified polyclonal antibody (Catalog # A12956-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOX2 at approximately 70 kDa. The expected band size for TOX2 is at 52 kDa.
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