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Western Blot analysis of 293 cells using PIG3 Polyclonal Antibody
Western Blot analysis of 293 cells using PIG3 Polyclonal Antibody
Western Blot analysis of 293 cells using PIG3 Polyclonal Antibody

TP53I3 Antibody

CSB-PA003784
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTP53I3
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Overview

  • Supplier
    Cusabio
  • Product Name
    TP53I3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9540
  • Target name
    TP53I3
  • Target description
    tumor protein p53 inducible protein 3
  • Target synonyms
    PIG3, quinone oxidoreductase PIG3, NADPH:quinone reductase PIG3, p53-induced gene 3 protein, quinone oxidoreductase homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ53FA7
  • Protein Name
    Quinone oxidoreductase PIG3
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PIG3/TP53I3 using anti-PIG3/TP53I3 antibody (A06870-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human T-47D whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIG3/TP53I3 antigen affinity purified polyclonal antibody (Catalog # A06870-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PIG3/TP53I3 at approximately 36 kDa. The expected band size for PIG3/TP53I3 is at 36 kDa.
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