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Western Blot analysis of HepG2 cells using TPH1 Polyclonal Antibody
Western Blot analysis of HepG2 cells using TPH1 Polyclonal Antibody
Western Blot analysis of HepG2 cells using TPH1 Polyclonal Antibody

TPH1 Antibody

CSB-PA004319
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTPH1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TPH1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7166
  • Target name
    TPH1
  • Target description
    tryptophan hydroxylase 1
  • Target synonyms
    indoleacetic acid-5-hydroxylase; L-tryptophan hydroxylase; TPRH; TRPH; tryptophan 5-hydroxylase 1; tryptophan 5-monooxygenase 1; tryptophan hydroxylase (tryptophan 5-monooxygenase)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17752
  • Protein Name
    Tryptophan 5-hydroxylase 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Tryptophan Hydroxylase using anti-Tryptophan Hydroxylase antibody (A01626-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SGC-7901 whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tryptophan Hydroxylase antigen affinity purified polyclonal antibody (Catalog # A01626-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tryptophan Hydroxylase at approximately 51KD. The expected band size for Tryptophan Hydroxylase is at 51KD.
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