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IHC-P analysis of human cerebellum using GTX88044 TPPP antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 2microg/ml
IHC-P analysis of human cerebellum using GTX88044 TPPP antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 2microg/ml
IHC-P analysis of human cerebellum using GTX88044 TPPP antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 2microg/ml

TPPP antibody, Internal

GTX88044
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTPPP
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Overview

  • Supplier
    GeneTex
  • Product Name
    TPPP antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.001-0.01microg/ml. IHC-P: 3.75microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID11076
  • Target name
    TPPP
  • Target description
    tubulin polymerization promoting protein
  • Target synonyms
    TPPP/p25, TPPP1, p24, p25, p25alpha, tubulin polymerization-promoting protein, 25 kDa brain-specific protein, brain specific protein p25 alpha, glycogen synthase kinase 3 (GSK3) inhibitor p24, p25-alpha
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDO94811
  • Protein Name
    Tubulin polymerization-promoting protein
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TPPP using anti-TPPP antibody (A04389-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPPP antigen affinity purified polyclonal antibody (Catalog # A04389-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPPP at approximately 24 kDa. The expected band size for TPPP is at 24 kDa.
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