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TPR antibody

GTX130142
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetTPR
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Overview

  • Supplier
    GeneTex
  • Product Name
    TPR antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.37 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7175
  • Target name
    TPR
  • Target description
    translocated promoter region, nuclear basket protein
  • Target synonyms
    megator; NPC-associated intranuclear protein; nuclear pore complex-associated protein TPR; nucleoprotein TPR; translocated promoter region (to activated MET oncogene); translocated promoter region protein; tumor potentiating region
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12270
  • Protein Name
    Nucleoprotein TPR
  • Scientific Description
    This gene encodes a large coiled-coil protein that forms intranuclear filaments attached to the inner surface of nuclear pore complexes (NPCs). The protein directly interacts with several components of the NPC. It is required for the nuclear export of mRNAs and some proteins. Oncogenic fusions of the 5 end of this gene with several different kinase genes occur in some neoplasias. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TPR using anti-TPR antibody (A00695-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPR antigen affinity purified polyclonal antibody (Catalog # A00695-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPR at approximately 300 kDa. The expected band size for TPR is at 265 kDa.
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