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Western Blot analysis of Jurkat cells using TPX2 Polyclonal Antibody
Western Blot analysis of Jurkat cells using TPX2 Polyclonal Antibody
Western Blot analysis of Jurkat cells using TPX2 Polyclonal Antibody

TPX2 Antibody

CSB-PA008292
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTPX2
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Overview

  • Supplier
    Cusabio
  • Product Name
    TPX2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID22974
  • Target name
    TPX2
  • Target description
    TPX2 microtubule nucleation factor
  • Target synonyms
    C20orf1, C20orf2, DIL-2, DIL2, FLS353, GD:C20orf1, HCA519, HCTP4, REPP86, p100, targeting protein for Xklp2, TPX2, microtubule-associated protein homolog, TPX2, microtubule-associated, homolog, differentially expressed in cancerous and non-cancerous lung cells 2, differentially expressed in cancerous and noncancerous lung cells 2, differentially expressed in lung cells, hepatocellular carcinoma-associated antigen 519, hepatocellular carcinoma-associated antigen 90, preferentially expressed in colorectal cancer, protein fls353, restricted expression proliferation associated protein 100
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9ULW0
  • Protein Name
    Targeting protein for Xklp2
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TPX2 using anti-TPX2 antibody (A01610-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human SGC-7901 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPX2 antigen affinity purified polyclonal antibody (Catalog # A01610-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPX2 at approximately 86KD, 100KD. The expected band size for TPX2 is at 86KD.
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