TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![TRAF6 antibody [N2C2], Internal immunoprecipitates TRAF6 protein in IP experiments. IP samples: K562 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of TRAF6 protein by 4 μg TRAF6 antibody [N2C2], Internal (GTX113029) 7.5 % SDS-PAGE The immunoprecipitated TRAF6 protein was detected by TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX113029/GTX113029_40093_IP_w_23060500_861.webp "TRAF6 antibody [N2C2], Internal immunoprecipitates TRAF6 protein in IP experiments. IP samples: K562 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of TRAF6 protein by 4 μg TRAF6 antibody [N2C2], Internal (GTX113029) 7.5 % SDS-PAGE The immunoprecipitated TRAF6 protein was detected by TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]")
![TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX113029/GTX113029_42452_20160615_IHC-P_R_w_23060500_600.webp "TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRAF6 protein stained by TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:100. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113029/GTX113029_40093_20150410_IFA_w_23060500_542.webp "TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRAF6 protein stained by TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:100. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining.")
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113029/GTX113029_45518_20241011_WB_25080802_809.webp "Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm in mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. TRAF6 antibody [N2C2], Internal (GTX113029) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
TRAF6 antibody [N2C2], Internal
GTX113029
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTRAF6
Overview
- SupplierGeneTex
- Product NameTRAF6 antibody [N2C2], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration3.39 mg/ml
- ConjugateUnconjugated
- Gene ID7189
- Target nameTRAF6
- Target descriptionTNF receptor associated factor 6
- Target synonymsMGC:3310, RNF85, TNF receptor-associated factor 6, E3 ubiquitin-protein ligase TRAF6, RING finger protein 85, RING-type E3 ubiquitin transferase TRAF6, TNF receptor-associated factor 6, E3 ubiquitin protein ligase, interleukin-1 signal transducer
- HostRabbit
- IsotypeIgG
- Protein IDQ9Y4K3
- Protein NameTNF receptor-associated factor 6
- Scientific DescriptionThe protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein mediates the signaling not only from the members of the TNF receptor superfamily, but also from the members of the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. Two alternatively spliced transcript variants encoding identical proteins have been reported. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Vos S, Aaron R, Weng M, et al. CD40 Upregulation in the Retina of Patients With Diabetic Retinopathy: Association With TRAF2/TRAF6 Upregulation and Inflammatory Molecule Expression. Invest Ophthalmol Vis Sci. 2023,64(7):17. doi: 10.1167/iovs.64.7.17Read this paper
- Han P, Sunada-Nara K, Kawashima N, et al. MicroRNA-146b-5p Suppresses Pro-Inflammatory Mediator Synthesis via Targeting TRAF6, IRAK1, and RELA in Lipopolysaccharide-Stimulated Human Dental Pulp Cells. Int J Mol Sci. 2023,24(8). doi: 10.3390/ijms24087433Read this paper
- Lim TJF, Bunjamin M, Ruedl C, et al. Talin1 controls dendritic cell activation by regulating TLR complex assembly and signaling. J Exp Med. 2020,217(8). doi: 10.1084/jem.20191810Read this paper
- Nara K, Kawashima N, Noda S, et al. Anti-inflammatory roles of microRNA 21 in lipopolysaccharide-stimulated human dental pulp cells. J Cell Physiol. 2019,234(11):21331-21341. doi: 10.1002/jcp.28737Read this paper
- Huang WC, Liao JH, Hsiao TC, et al. Binding and Enhanced Binding between Key Immunity Proteins TRAF6 and TIFA. Chembiochem. 2019,20(2):140-146. doi: 10.1002/cbic.201800436Read this paper
- Zhong X, Liao Y, Chen L, et al. The MicroRNAs in the Pathogenesis of Metabolic Memory. Endocrinology. 2015,156(9):3157-68. doi: 10.1210/en.2015-1063Read this paper
- Hung CJ, Kao CT, Chen YJ, et al. Antiosteoclastogenic activity of silicate-based materials antagonizing receptor activator for nuclear factor kappaB ligand-induced osteoclast differentiation of murine marcophages. J Endod. 2013,39(12):1557-61. doi: 10.1016/j.joen.2013.07.004Read this paper
Datasheet
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