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TRAF7 Antibody

LS-C332355
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTRAF7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TRAF7 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 66kDa/74kDa, while the observed MW by Western blot was 70kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:100), WB (1:500 - 1:1000) The predicted MW is 66kDa/74kDa, while the observed MW by Western blot was 70kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.689 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID84231
  • Target name
    TRAF7
  • Target description
    TNF receptor associated factor 7
  • Target synonyms
    CAFDADD, RFWD1, RNF119, E3 ubiquitin-protein ligase TRAF7, RING finger and WD repeat-containing protein 1, RING finger protein 119, RING-type E3 ubiquitin transferase TRAF7, TNF receptor-associated factor 7, E3 ubiquitin protein ligase, ring finger and WD repeat domain 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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IHC image of CSB-PA757671LA01HU diluted at 1:200 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of TRAF7 using anti-TRAF7 antibody (A05972-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF7 antigen affinity purified polyclonal antibody (Catalog # A05972-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF7 at approximately 75 kDa. The expected band size for TRAF7 is at 75 kDa.
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