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TRAIP Polyclonal Antibody

RD79535A
Reddot Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTRAIP
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    TRAIP Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.8 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10293
  • Target name
    TRAIP
  • Target description
    TRAF interacting protein
  • Target synonyms
    E3 ubiquitin-protein ligase TRAIP; ring finger protein 206; RING-type E3 ubiquitin transferase TRAIP; RNF206; SCKL9; TRIP
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes a protein that contains an N-terminal RING finger motif and a putative coiled-coil domain. A similar murine protein interacts with TNFR-associated factor 1 (TRAF1), TNFR-associated factor 2 (TRAF2), and cylindromatosis. The interaction with TRAF2 inhibits TRAF2-mediated nuclear factor kappa-B, subunit 1 activation that is required for cell activation and protection against apoptosis.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRIP/TRAIP using anti-TRIP/TRAIP antibody (A07953-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIP/TRAIP antigen affinity purified polyclonal antibody (Catalog # A07953-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIP/TRAIP at approximately 53 kDa. The expected band size for TRIP/TRAIP is at 53 kDa.
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