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WB analysis of human fetal liver tissue using GTX45662 TRAM1L1 antibody at 0.2-1microg/ml.
WB analysis of human fetal liver tissue using GTX45662 TRAM1L1 antibody at 0.2-1microg/ml.
WB analysis of human fetal liver tissue using GTX45662 TRAM1L1 antibody at 0.2-1microg/ml.

TRAM1L1 antibody, Internal

GTX45662
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetTRAM1L1
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Overview

  • Supplier
    GeneTex
  • Product Name
    TRAM1L1 antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID133022
  • Target name
    TRAM1L1
  • Target description
    translocation associated membrane protein 1 like 1
  • Target synonyms
    translocating chain-associated membrane protein 1-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N609
  • Protein Name
    Translocating chain-associated membrane protein 1-like 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRAM1L1 using anti-TRAM1L1 antibody (A18207-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAM1L1 antigen affinity purified polyclonal antibody (Catalog # A18207-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAM1L1 at approximately 52 kDa. The expected band size for TRAM1L1 is at 42 kDa.
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