Sample (50 μg of whole cell lysate) A: mouse brain 12% SDS PAGE GTX115082 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX115082/GTX115082_41059_20170417_IHC-P_M_1_w_23060519_197.webp "Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Transgelin 2 protein stained by Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX115082/GTX115082_40261_20150410_IFA_w_23060519_560.webp "Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Transgelin 2 protein stained by Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200. Blue: Hoechst 33342 staining.")
![Wild-type (WT) and Transgelin 2 knockout (KO) 293T cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX115082/GTX115082_40261_20190322_WB_KO_watermark_w_23060519_518.webp "Wild-type (WT) and Transgelin 2 knockout (KO) 293T cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in ice-cold MeOH for 5 min. Green: Transgelin 2 protein stained by Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX115082/GTX115082_41059_IFA_w_23060519_974.webp "Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in ice-cold MeOH for 5 min. Green: Transgelin 2 protein stained by Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.")
![Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX115082/GTX115082_41059_20170417_IHC-P_M_2_w_23060519_166.webp "Transgelin 2 antibody [N1C3] detects Transgelin 2 protein at cytoplasm in mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX115082/GTX115082_40261_20250124_WB_TPM_watermark_25020422_213.webp "Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin 2 antibody [N1C3] (GTX115082) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
Sample (50 μg of whole cell lysate) A: mouse brain 12% SDS PAGE GTX115082 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Transgelin 2 antibody [N1C3]
GTX115082
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetTAGLN2
Overview
- SupplierGeneTex
- Product NameTransgelin 2 antibody [N1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.53 mg/ml
- ConjugateUnconjugated
- Gene ID8407
- Target nameTAGLN2
- Target descriptiontransgelin 2
- Target synonymsHA1756, transgelin-2, SM22-alpha homolog, epididymis secretory sperm binding protein, epididymis tissue protein Li 7e
- HostRabbit
- IsotypeIgG
- Protein IDP37802
- Protein NameTransgelin-2
- Scientific DescriptionThe protein encoded by this gene is a homolog of the protein transgelin, which is one of the earliest markers of differentiated smooth muscle. The function of this protein has not yet been determined. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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