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Rat Liver lysates probed with TREM1 Polyclonal Antibody, Unconjugated (bs-4886R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Rat Liver lysates probed with TREM1 Polyclonal Antibody, Unconjugated (bs-4886R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Rat Liver lysates probed with TREM1 Polyclonal Antibody, Unconjugated (bs-4886R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

TREM1 Polyclonal Antibody

BS-4886R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse, Rat
TargetTrem1
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Overview

  • Supplier
    Bioss
  • Product Name
    TREM1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID58217
  • Target name
    Trem1
  • Target description
    triggering receptor expressed on myeloid cells 1
  • Target synonyms
    triggering receptor expressed on myeloid cells 1, triggering receptor expressed in monocytes 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9JKE2
  • Protein Name
    Triggering receptor expressed on myeloid cells 1
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Teng Y, Feng C, Liu Y, et al. Anti-inflammatory effect of tranexamic acid against trauma-hemorrhagic shock-induced acute lung injury in rats. Exp Anim. 2018,67(3):313-320. doi: 10.1538/expanim.17-0143
    Read this paper
  • Zhao Y, Guo Y, Jiang Y, et al. Vitamin D suppresses macrophage infiltration by down-regulation of TREM-1 in diabetic nephropathy rats. Mol Cell Endocrinol. 2018,473:44-52. doi: 10.1016/j.mce.2018.01.001
    Read this paper

Datasheet

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Figure 1. Western blot analysis of TREM1 using anti-TREM1 antibody (A02135-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: rat spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREM1 antigen affinity purified polyclonal antibody (Catalog # A02135-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TREM1 at approximately 26KD. The expected band size for TREM1 is at 26KD.
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