TREM2 antibody [HL1738] detects TREM2 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TREM2 stained by TREM2 antibody [HL1738] (GTX637386) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1738] (GTX637386) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637386/GTX637386_44844_20221125_WB_B_22112723_805.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1738] (GTX637386) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![TREM2 antibody [HL1738] detects TREM2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TREM2 stained by TREM2 antibody [HL1738] (GTX637386) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637386/GTX637386_T-44781_20221118_IHC-P_22120719_618.webp "TREM2 antibody [HL1738] detects TREM2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. TREM2 stained by TREM2 antibody [HL1738] (GTX637386) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human TREM2 protein (1215.95-19.07 pM). Coated protein was probed with TREM2 antibody [HL1738] (GTX637386) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637386/GTX637386_44844_20231117_ELISA_Indirect_23112021_255.webp "Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length human TREM2 protein (1215.95-19.07 pM). Coated protein was probed with TREM2 antibody [HL1738] (GTX637386) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.")
![Sandwich ELISA detection of recombinant E.coli expressed, full-length human TREM2 protein using antibodies as below. Capture: TREM2 antibody [HL1739] (GTX637387) (5 μg/mL) Detection: HRP-conjugated TREM2 antibody [HL1738] (GTX637386) (1 μg/mL) Please notice that GTX637386 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637387. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637386/GTX637386_44844_20231124_ELISA_PAIR_2_23112922_700.webp "Sandwich ELISA detection of recombinant E.coli expressed, full-length human TREM2 protein using antibodies as below. Capture: TREM2 antibody [HL1739] (GTX637387) (5 μg/mL) Detection: HRP-conjugated TREM2 antibody [HL1738] (GTX637386) (1 μg/mL) Please notice that GTX637386 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637387. Please contact us for custom HRP-conjugated antibody.")
![Sandwich ELISA detection of recombinant E.coli expressed, full-length human TREM2 protein using antibodies as below. Capture: TREM2 antibody [HL1738] (GTX637386) (5 μg/mL) Detection: HRP-conjugated TREM2 antibody [HL1739] (GTX637387) (1 μg/mL) Please notice that GTX637387 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637386. Please contact us for custom HRP-conjugated antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637386/GTX637386_44844_20231124_ELISA_PAIR_23112922_878.webp "Sandwich ELISA detection of recombinant E.coli expressed, full-length human TREM2 protein using antibodies as below. Capture: TREM2 antibody [HL1738] (GTX637386) (5 μg/mL) Detection: HRP-conjugated TREM2 antibody [HL1739] (GTX637387) (1 μg/mL) Please notice that GTX637387 needs to be conjugated to HRP to function as the detection antibody when paired with GTX637386. Please contact us for custom HRP-conjugated antibody.")
![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1738] (GTX637386) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637386/GTX637386_45278_20240126_WB_TPM_watermark_25073123_310.webp "Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1738] (GTX637386) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
TREM2 antibody [HL1738] detects TREM2 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TREM2 stained by TREM2 antibody [HL1738] (GTX637386) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
TREM2 antibody [HL1738]
GTX637386
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTREM2
Overview
- SupplierGeneTex
- Product NameTREM2 antibody [HL1738]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1738
- Concentration2 mg/ml
- ConjugateUnconjugated
- Gene ID54209
- Target nameTREM2
- Target descriptiontriggering receptor expressed on myeloid cells 2
- Target synonymsAD17, PLOSL2, TREM-2, Trem2a, Trem2b, Trem2c, triggering receptor expressed on myeloid cells 2, triggering receptor expressed on monocytes 2, triggering receptor expressed on myeloid cells 2a
- HostRabbit
- IsotypeIgG
- Protein IDQ9NZC2
- Protein NameTriggering receptor expressed on myeloid cells 2
- Scientific DescriptionThis gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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![Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with TREM2 antibody [HL1739] (GTX637387) diluted at 1:250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637387/GTX637387_T-44781_20221118_WB_treatment_PNGaseF_22112219_839.webp)
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