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ProductsBack/TREX2 Antibody - C-terminal region (OAAB19042)

TREX2 Antibody - C-terminal region (OAAB19042)

OAAB19042
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetTREX2
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    TREX2 Antibody - C-terminal region (OAAB19042)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Format
    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
  • Gene ID11219
  • Target name
    TREX2
  • Target description
    three prime repair exonuclease 2
  • Target synonyms
    3'-5' exonuclease TREX2 long form; three prime repair exonuclease 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TREX2 using anti-TREX2 antibody (PB10105). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: MCF-7 whole cell lysates lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREX2 antigen affinity purified polyclonal antibody (Catalog # PB10105) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TREX2 at approximately 37 kDa. The expected band size for TREX2 is at 31 kDa.
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