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TRF2 antibody

GTX132780
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetTERF2
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Overview

  • Supplier
    GeneTex
  • Product Name
    TRF2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.47 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7014
  • Target name
    TERF2
  • Target description
    telomeric repeat binding factor 2
  • Target synonyms
    telomeric DNA-binding protein; telomeric repeat binding protein 2; telomeric repeat-binding factor 2; TRBF2; TRF2; TTAGGG repeat-binding factor 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15554
  • Protein Name
    Telomeric repeat-binding factor 2
  • Scientific Description
    This gene encodes a telomere specific protein, TERF2, which is a component of the telomere nucleoprotein complex. This protein is present at telomeres in metaphase of the cell cycle, is a second negative regulator of telomere length and plays a key role in the protective activity of telomeres. While having similar telomere binding activity and domain organization, TERF2 differs from TERF1 in that its N terminus is basic rather than acidic. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRF2 using anti-TRF2 antibody (A00650-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: human COLO320 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRF2 antigen affinity purified polyclonal antibody (Catalog # A00650-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRF2 at approximately 75 kDa. The expected band size for TRF2 is at 59KD.
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Immunohistochemical staining of human cerebral cortex, gastrointestinal, lymphoid tissues and testis using Anti-TERF2 antibody HPA001907 (A) shows similar protein distribution across tissues to independent antibody HPA002735 (B).
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FACS analysis of Jurkat cells using GTX13579 TRF2 antibody [4A794.15]. Red : Primary antibody Blue : isotype control Antibody amount : 0.1microg
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