Bio-Connect

Trident Universal Protein Blocking Reagent (animal serum free)

Research Use Only
GTX30963
GeneTex
Product group Technique Specific Products
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Overview

  • Supplier
    GeneTex
  • Product Name
    Trident Universal Protein Blocking Reagent (animal serum free)
  • Delivery Days Customer
    9
  • Application Supplier Note
    Ready to use. Western blot: Immerse the filter in a sufficient amount of Blocking Reagent and incubate for 30 minutes at RT with gentle shaking. IHC/ICC: Incubate tissue sections/ cell smears in Blocking Reagent for 10 minutes at RT or 37C. Note: Optimal incubation times should be determined by the researcher.
  • Certification
    Research Use Only
  • Formulation
    Liquid
  • Scientific Description
    Immunohistochemistry (IHC)/ Immunocytochemistry (ICC) is the localization of antigens by the use of antigens in tissue sections/cells by the use of labeled antibodies as specific reagents through antigen-antibody interactions that are visualized by a marker such as fluorescent dye, enzyme, radioactive element or colloidal gold. Several IHC techniques are commonly used: labeled biotin secondary antibody streptavidin-peroxidase (LBSASP), HRP anti-HRP, ABC, catalyzed signal amplification, polymer system and others, to detect antigens on tissue and cell In this kit the first layer is unlabeled primary antibody, the second layer is biotinylated secondary antibody, the third layer is Enzyme-Streptavidin conjugate (HRP-Streptavidin) to replace the complex of avidin-biotin peroxidase. The enzyme is then visualized by application of the substrate chromogen solution to produce different colorimetric end products. This protein blocking solution does not contain any phosphate, therefore it is good for alkaline phosphatase assays.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41105300

References

  • Discovery of Potential Neuroprotective Agents against Paclitaxel-Induced Peripheral Neuropathy. Chen YF et al., 2022 Mar 24, J Med Chem
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  • Differential expression of hypoxia-inducible factors related to the invasiveness of epithelial ovarian cancer. Shih HJ et al., 2021 Nov 25, Sci Rep
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  • miRNA-148a Enhances the Treatment Response of Patients with Rectal Cancer to Chemoradiation and Promotes Apoptosis by Directly Targeting c-Met. Huang CM et al., 2021 Oct 1, Biomedicines
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  • Identifying cell-enriched miRNAs in kidney injury and repair. Connor KL et al., 2020 Dec 17, JCI Insight
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  • Circulating Levels of Epirubicin Cause Endothelial Senescence While Compromising Metabolic Activity and Vascular Function. Eakin AJ et al., 2020, Front Cell Dev Biol
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  • Single-Cell Transcriptomics Uncovers Zonation of Function in the Mesenchyme during Liver Fibrosis. Dobie R et al., 2019 Nov 12, Cell Rep
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  • Resolving the fibrotic niche of human liver cirrhosis at single-cell level. Ramachandran P et al., 2019 Nov, Nature
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  • Pterostilbene modulates the suppression of multidrug resistance protein 1 and triggers autophagic and apoptotic mechanisms in cisplatin-resistant human oral cancer CAR cells via AKT signaling. Chang HP et al., 2018 Mar 2, Int J Oncol
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  • Recruitment of CD34(+) fibroblasts in tumor-associated reactive stroma: the reactive microvasculature hypothesis. San Martin R et al., 2014 Jun, Am J Pathol
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