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ProductsBack/TRIM antibody [TRIM-04] (PE)

TRIM antibody [TRIM-04] (PE)

GTX79872
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetTRAT1
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Overview

  • Supplier
    GeneTex
  • Product Name
    TRIM antibody [TRIM-04] (PE)
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    TRIM-04
  • Concentration
    0.1 mg/ml
  • Conjugate
    RPE
  • Gene ID50852
  • Target name
    TRAT1
  • Target description
    T cell receptor associated transmembrane adaptor 1
  • Target synonyms
    HSPC062, TCRIM, TRIM, pp29/30, T-cell receptor-associated transmembrane adapter 1, T cell receptor interacting molecule
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ6PIZ9
  • Protein Name
    T-cell receptor-associated transmembrane adapter 1
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRAT1 using anti-TRAT1 antibody (A11223). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAT1 antigen affinity purified polyclonal antibody (Catalog # A11223) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAT1 at approximately 27-30 kDa. The expected band size for TRAT1 is at 21 kDa.
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