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WB analysis of rat brain lysate using GTX89871 TRIM2 antibody, C-term. Dilution : 0.5microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of rat brain lysate using GTX89871 TRIM2 antibody, C-term. Dilution : 0.5microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of rat brain lysate using GTX89871 TRIM2 antibody, C-term. Dilution : 0.5microg/ml Loading : 35microg protein in RIPA buffer

TRIM2 antibody, C-term

GTX89871
GeneTex
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTRIM2
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Overview

  • Supplier
    GeneTex
  • Product Name
    TRIM2 antibody, C-term
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 0.5-2microg/ml. IHC-P: 6-12microg/ml. FACS: 10 microg/ml. ELISA: <1:16000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23321
  • Target name
    TRIM2
  • Target description
    tripartite motif containing 2
  • Target synonyms
    CMT2R; E3 ubiquitin-protein ligase TRIM2; RING finger protein 86; RING-type E3 ubiquitin transferase TRIM2; RNF86; tripartite motif protein TRIM2; tripartite motif-containing protein 2
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ9C040
  • Protein Name
    Tripartite motif-containing protein 2
  • Scientific Description
    The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. It plays a neuroprotective role and functions as an E3-ubiquitin ligase in proteasome-mediated degradation of target proteins. Mutations in this gene can cause early-onset axonal neuropathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRIM2 using anti-TRIM2 antibody (A08957). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM2 antigen affinity purified polyclonal antibody (Catalog # A08957) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM2 at approximately 82 kDa. The expected band size for TRIM2 is at 82 kDa.
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