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Goat anti-TRIM2

EB05914
Everest Biotech
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTRIM2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-TRIM2 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID23321
  • Target name
    TRIM2
  • Target description
    tripartite motif containing 2
  • Target synonyms
    CMT2R; E3 ubiquitin-protein ligase TRIM2; RING finger protein 86; RING-type E3 ubiquitin transferase TRIM2; RNF86; tripartite motif protein TRIM2; tripartite motif-containing protein 2
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_056086.2; NP_001123539.1; NP_001337983.1; NP_001337984.1; NP_001337985.1l; NP_001337986.1. GeneIDs all Nonhuman: 80890 (mouse);. Purification: Antigen affinity purified. Names and symbols: TRIM2; tripartite motif-containing 2; KIAA0517; tripartite motif protein 2; tripartite motif protein TRIM2; RNF86; tripartite motif protein TRIM2
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRIM2 using anti-TRIM2 antibody (A08957). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM2 antigen affinity purified polyclonal antibody (Catalog # A08957) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM2 at approximately 82 kDa. The expected band size for TRIM2 is at 82 kDa.
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Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
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