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Western Blot analysis of Lovo, A549 and hela cell using TRIM21 Polyclonal Antibody at dilution of 1:400
Western Blot analysis of Lovo, A549 and hela cell using TRIM21 Polyclonal Antibody at dilution of 1:400
Western Blot analysis of Lovo, A549 and hela cell using TRIM21 Polyclonal Antibody at dilution of 1:400

TRIM21 Polyclonal Antibody

E-AB-15410
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    TRIM21 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB
  • Certification
    Research Use Only
  • Concentration
    0.4mg/ml
  • Scientific Description
    This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus.
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of TRIM21/SS-A using anti-TRIM21/SS-A antibody (A02079-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: mouse lung tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: mouse thymus tissue lysates, Lane 5: human Hela whole cell lysates, Lane 6: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM21/SS-A antigen affinity purified polyclonal antibody (Catalog # A02079-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM21/SS-A at approximately 52KD. The expected band size for TRIM21/SS-A is at 52KD.
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