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Western Blot analysis of COLO205 cells using TIF1alpha Polyclonal Antibody
Western Blot analysis of COLO205 cells using TIF1alpha Polyclonal Antibody
Western Blot analysis of COLO205 cells using TIF1alpha Polyclonal Antibody

TRIM24 Antibody

CSB-PA004291
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTRIM24
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Overview

  • Supplier
    Cusabio
  • Product Name
    TRIM24 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8805
  • Target name
    TRIM24
  • Target description
    tripartite motif containing 24
  • Target synonyms
    E3 ubiquitin-protein ligase TRIM24; hTIF1; PTC6; RING finger protein 82; RING-type E3 ubiquitin transferase TIF1-alpha; RNF82; TF1A; TIF1; TIF1A; TIF1ALPHA; TIF1-alpha; transcription intermediary factor 1-alpha; transcriptional intermediary factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15164
  • Protein Name
    Transcription intermediary factor 1-alpha
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRIM24 using anti-TRIM24 antibody (A03258-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM24 antigen affinity purified polyclonal antibody (Catalog # A03258-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM24 at approximately 130KD. The expected band size for TRIM24 is at 117KD.
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