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TRIM25 antibody

70R-20982
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    TRIM25 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRIM25 using anti-TRIM25 antibody (A03232-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human T-47D whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM25 antigen affinity purified polyclonal antibody (Catalog # A03232-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM25 at approximately 71KD. The expected band size for TRIM25 is at 71KD.
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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA054075(TRIM25 Antibody) at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM25 antibody [N2C1], Internal (GTX100617) diluted at 1:500.
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Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
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