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Western Blot analysis of COLO cells using TRIM3 Polyclonal Antibody
Western Blot analysis of COLO cells using TRIM3 Polyclonal Antibody
Western Blot analysis of COLO cells using TRIM3 Polyclonal Antibody

TRIM3 Antibody

CSB-PA006667
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTRIM3
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Overview

  • Supplier
    Cusabio
  • Product Name
    TRIM3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10612
  • Target name
    TRIM3
  • Target description
    tripartite motif containing 3
  • Target synonyms
    BERP, HAC1, RNF22, RNF97, tripartite motif-containing protein 3, RING finger protein 97, brain expressed ring finger, brain-expressed RING finger protein, ring finger protein 22, tripartite motif protein TRIM3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75382
  • Protein Name
    Tripartite motif-containing protein 3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRIM3 using anti-TRIM3 antibody (A07081-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM3 antigen affinity purified polyclonal antibody (Catalog # A07081-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM3 at approximately 81 kDa. The expected band size for TRIM3 is at 81 kDa.
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