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ProductsBack/TRIM50 / E3 Ubiquitin Ligase Antibody (aa354-383)

TRIM50 / E3 Ubiquitin Ligase Antibody (aa354-383)

LS-C330255
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTRIM50
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TRIM50 / E3 Ubiquitin Ligase Antibody (aa354-383)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:1000), Flo (1:10 - 1:50), IHC (1:50 - 1:100), WB (1:100 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID135892
  • Target name
    TRIM50
  • Target description
    tripartite motif containing 50
  • Target synonyms
    TRIM50A, E3 ubiquitin-protein ligase TRIM50, RING-type E3 ubiquitin transferase TRIM50, tripartite motif-containing 50A, tripartite motif-containing protein 50
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRIM50 using anti-TRIM50 antibody (A14090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM50 antigen affinity purified polyclonal antibody (Catalog # A14090-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM50 at approximately 55 kDa. The expected band size for TRIM50 is at 55 kDa.
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