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TRIM72 Antibody

ORB1249020
Biorbyt
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Rat
TargetTRIM72
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Overview

  • Supplier
    Biorbyt
  • Product Name
    TRIM72 Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 52kDa band observed in Mouse Heart lysates (calculated MW of 52.8kDa according to Mouse NP_001073401.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows staining of the fibre membranes, detached artificially by the sectioning. Recommended concentration: 3-5ug/ml.. Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 52kDa band observed in Mouse Heart lysates (calculated MW of 52.8kDa according to Mouse NP_001073401.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows staining of the fibre membranes, detached artificially by the sectioning. Recommended concentration: 3-5ug/ml. ELISA, IHC, WB
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID493829
  • Target name
    TRIM72
  • Target description
    tripartite motif containing 72
  • Target synonyms
    MG53, tripartite motif-containing protein 72, mitsugumin-53, tripartite motif containing 72, E3 ubiquitin protein ligase
  • Host
    Goat
  • Protein IDQ6ZMU5
  • Protein Name
    Tripartite motif-containing protein 72
  • Scientific Description
    TRIM72 Antibody
  • Reactivity
    Bovine, Canine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MG53/TRIM72 using anti-MG53/TRIM72 antibody (A06982-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse skeletal muscle tissue lysates, Lane 4: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MG53/TRIM72 antigen affinity purified polyclonal antibody (Catalog # A06982-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MG53/TRIM72 at approximately 55 kDa. The expected band size for MG53/TRIM72 is at 53 kDa.
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