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ProductsBack/TRIP13 Antibody (HRP)

TRIP13 Antibody (HRP)

LS-C377542
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetTRIP13
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TRIP13 Antibody (HRP)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ELISA
  • Applications Supplier
    ELISA The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID9319
  • Target name
    TRIP13
  • Target description
    thyroid hormone receptor interactor 13
  • Target synonyms
    16E1BP; 16E1-BP; HPV16 E1 protein binding protein; human papillomavirus type 16 E1 protein-binding protein; MVA3; OOMD9; pachytene checkpoint protein 2 homolog; SH3TC1/TRIP13 fusion; thyroid receptor-interacting protein 13; TR-interacting protein 13; TRIP-13
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PCH2/TRIP13 using anti-PCH2/TRIP13 antibody (A06921-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCH2/TRIP13 antigen affinity purified polyclonal antibody (Catalog # A06921-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCH2/TRIP13 at approximately 49 kDa. The expected band size for PCH2/TRIP13 is at 49 kDa.
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