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Western Blot analysis of A431 cell using TRIP4 Polyclonal Antibody at dilution of 1:400
Western Blot analysis of A431 cell using TRIP4 Polyclonal Antibody at dilution of 1:400
Western Blot analysis of A431 cell using TRIP4 Polyclonal Antibody at dilution of 1:400

TRIP4 Polyclonal Antibody

E-AB-14750
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    TRIP4 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.6mg/ml
  • Scientific Description
    Activating signal co-integrator-1 (ASC-1), originally identified as TRIP4, is a transcriptional co-activator of nuclear receptors that associates with specific components of the RNA polymerase II complex and binds the basal transcription factors TBP and TFIIA. ASC-1 functions with the transcription integrators SRC-1 and CBP/p300 through its zinc finger motif and is dependent on their ligand-dependent transactivation domain, AF2. Endogenous ASC-1 in HeLa cells is predominantly a nuclear protein. Under conditions of serum starvation, ASC-1 localizes to the cytoplasm.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ASC1/TRIP4 using anti-ASC1/TRIP4 antibody (A07762-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASC1/TRIP4 antigen affinity purified polyclonal antibody (Catalog # A07762-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASC1/TRIP4 at approximately 68 kDa. The expected band size for ASC1/TRIP4 is at 66 kDa.
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