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ProductsBack/TrkC (NTRK3) Mouse Monoclonal Antibody [Clone ID: OTI24B3]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NTRK3 (RC203781, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTRK3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NTRK3 (RC203781, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTRK3.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NTRK3 (RC203781, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTRK3.

TrkC (NTRK3) Mouse Monoclonal Antibody [Clone ID: OTI24B3]

CF500536
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNTRK3
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    TrkC (NTRK3) Mouse Monoclonal Antibody [Clone ID: OTI24B3]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI24B3
  • Gene ID4916
  • Target name
    NTRK3
  • Target description
    neurotrophic receptor tyrosine kinase 3
  • Target synonyms
    GP145-TrkC, TRKC, gp145(trkC), NT-3 growth factor receptor, ETS related protein-neurotrophic receptor tyrosine kinase fusion protein, ETV6-NTRK3 fusion, neurotrophic tyrosine kinase, receptor, type 3, tyrosine kinase receptor C
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ16288
  • Protein Name
    NT-3 growth factor receptor
  • Scientific Description
    Carrier-free (BSA/glycerol-free) NTRK3 mouse monoclonal antibody, clone OTI24B3 (formerly 24B3)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of TrkC/NTRK3 using anti-TrkC/NTRK3 antibody (A02502-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TrkC/NTRK3 antigen affinity purified polyclonal antibody (Catalog # A02502-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TrkC/NTRK3 at approximately 130 kDa. The expected band size for TrkC/NTRK3 is at 130 kDa.
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