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TRMT2B Antibody

LS-C668566
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetTRMT2B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TRMT2B Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western blot analysis of TRMT2B expression in SW480 (A), MCF7 (B) whole cell lysates.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Western blot analysis of TRMT2B expression in SW480 (A), MCF7 (B) whole cell lysates.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID79979
  • Target name
    TRMT2B
  • Target description
    tRNA methyltransferase 2 homolog B
  • Target synonyms
    CXorf34; dJ341D10.3; TRM2 homolog; TRM2 tRNA methyltransferase 2 homolog B; tRNA (uracil(54)-C(5))-methyltransferase homolog; tRNA (uracil-5-)-methyltransferase homolog
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRMT2B using anti-TRMT2B antibody (A16400-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT2B antigen affinity purified polyclonal antibody (Catalog # A16400-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRMT2B at approximately 47 kDa. The expected band size for TRMT2B is at 56 kDa.
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