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ProductsBack/TRMT61A / TRM61 Antibody

TRMT61A / TRM61 Antibody

LS-C497307
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetTRMT61A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TRMT61A / TRM61 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 31kDa, while the observed MW by Western blot was 31kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:2000) The predicted MW is 31kDa, while the observed MW by Western blot was 31kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID115708
  • Target name
    TRMT61A
  • Target description
    tRNA methyltransferase 61A
  • Target synonyms
    C14orf172, GCD14, Gcd14p, TRM61, hTRM61, tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A, mRNA methyladenosine-N(1)-methyltransferase catalytic subunit TRMT61A, tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRM61, tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRMT61A, tRNA methyltransferase 61 homolog A, tRNA(m1A58)-methyltransferase subunit TRM61, tRNA(m1A58)-methyltransferase subunit TRMT61A, tRNA(m1A58)MTase subunit TRM61, tRNA(m1A58)MTase subunit TRMT61A
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRMT61A using anti-TRMT61A antibody (A14512-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRMT61A antigen affinity purified polyclonal antibody (Catalog # A14512-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRMT61A at approximately 31 kDa. The expected band size for TRMT61A is at 31 kDa.
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