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Western blot All lanes: TRNAU1AP antibody at 12ug/ml Lane 1: MCF-7 whole cell lysate Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 33, 21 kDa Observed band size: 33 kDa
Western blot All lanes: TRNAU1AP antibody at 12ug/ml Lane 1: MCF-7 whole cell lysate Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 33, 21 kDa Observed band size: 33 kDa
Western blot All lanes: TRNAU1AP antibody at 12ug/ml Lane 1: MCF-7 whole cell lysate Lane 2: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 33, 21 kDa Observed band size: 33 kDa

TRNAU1AP Antibody

CSB-PA882127LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTRNAU1AP
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Overview

  • Supplier
    Cusabio
  • Product Name
    TRNAU1AP Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54952
  • Target name
    TRNAU1AP
  • Target description
    tRNA selenocysteine 1 associated protein 1
  • Target synonyms
    PRO1902, SECP43, TRSPAP1, tRNA selenocysteine 1-associated protein 1, tRNA selenocysteine associated protein (SECP43), tRNA selenocysteine-associated protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NX07
  • Protein Name
    tRNA selenocysteine 1-associated protein 1
  • Scientific Description
    Involved in the early steps of selenocysteine biosynthesis and tRNA(Sec) charging to the later steps resulting in the cotranslational incorporation of selenocysteine into selenoproteins. Stabilizes the SECISBP2, EEFSEC and tRNA(Sec) complex. May be involved in the methylation of tRNA(Sec). Enhances efficiency of selenoproteins synthesis (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of TRNAU1AP using anti-TRNAU1AP antibody (A15424-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRNAU1AP antigen affinity purified polyclonal antibody (Catalog # A15424-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRNAU1AP at approximately 40 kDa. The expected band size for TRNAU1AP is at 32 kDa.
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