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Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TRP12 Polyclonal Antibody, Unconjugated (bs-6425R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TRP12 Polyclonal Antibody, Unconjugated (bs-6425R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with TRP12 Polyclonal Antibody, Unconjugated (bs-6425R) at 1:400 overnight at 4°C, DAB staining.

TRP12/TRPV4 Polyclonal Antibody

BS-6425R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTRPV4
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Overview

  • Supplier
    Bioss
  • Product Name
    TRP12 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(), Flow-Cyt(1ug/Test), ICC/IF(1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID59341
  • Target name
    TRPV4
  • Target description
    transient receptor potential cation channel subfamily V member 4
  • Target synonyms
    BCYM3, CMT2C, HMSN2C, OTRPC4, SMAL, SPSMA, SSQTL1, TRP12, VRL2, VROAC, transient receptor potential cation channel subfamily V member 4, OSM9-like transient receptor potential channel 4, osm-9-like TRP channel 4, osmosensitive transient receptor potential channel 4, transient receptor potential protein 12, vanilloid receptor-like channel 2, vanilloid receptor-related osmotically activated channel
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HBA0
  • Protein Name
    Transient receptor potential cation channel subfamily V member 4
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Upregulated Expression of Transient Receptor Potential Cation Channel Subfamily V Receptors in Mucosae of Patients with Oral Squamous Cell Carcinoma and Patients with a History of Alcohol Consumption or Smoking. Sakakibara A et al., 2017, PLoS One
    Read more
  • Reduced tissue osmolarity increases TRPV4 expression and pro-inflammatory cytokines in intervertebral disc cells. Walter BA et al., 2016 Jul 19, Eur Cell Mater
    Read more

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Figure 1. Western blot analysis of TRPV4 using anti-TRPV4 antibody (A00565-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV4 antigen affinity purified polyclonal antibody (Catalog # A00565-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPV4 at approximately 98KD. The expected band size for TRPV4 is at 98KD.
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