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Western Blot analysis of HeLa cells using TRPC3 Polyclonal Antibody
Western Blot analysis of HeLa cells using TRPC3 Polyclonal Antibody
Western Blot analysis of HeLa cells using TRPC3 Polyclonal Antibody

TRPC3 Antibody

CSB-PA006033
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTRPC3
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Overview

  • Supplier
    Cusabio
  • Product Name
    TRPC3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7222
  • Target name
    TRPC3
  • Target description
    transient receptor potential cation channel subfamily C member 3
  • Target synonyms
    SCA41, TRP3, short transient receptor potential channel 3, hTrp-3, transient receptor protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13507
  • Protein Name
    Short transient receptor potential channel 3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRPC3 using anti-TRPC3 antibody (A01472-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat C6 whole cell lysates, Lane 2: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC3 antigen affinity purified polyclonal antibody (Catalog # A01472-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPC3 at approximately 105KD. The expected band size for TRPC3 is at 105KD.
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