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TRPC4 Antibody

CSB-PA025078GA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTRPC4
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Overview

  • Supplier
    Cusabio
  • Product Name
    TRPC4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7223
  • Target name
    TRPC4
  • Target description
    transient receptor potential cation channel subfamily C member 4
  • Target synonyms
    HTRP-4, HTRP4, TRP4, short transient receptor potential channel 4, trp-related protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBN4
  • Protein Name
    Short transient receptor potential channel 4
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRPC4 using anti-TRPC4 antibody (A02037-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human COLO-320 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC4 antigen affinity purified polyclonal antibody (Catalog # A02037-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPC4 at approximately 150KD. The expected band size for TRPC4 is at 112KD.
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