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TRPC5 Antibody

LS-C496802
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetTRPC5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TRPC5 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 111kDa, while the observed MW by Western blot was 115kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 111kDa, while the observed MW by Western blot was 115kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7224
  • Target name
    TRPC5
  • Target description
    transient receptor potential cation channel subfamily C member 5
  • Target synonyms
    PPP1R159, TRP5, short transient receptor potential channel 5, TRP-5, hTRP5, protein phosphatase 1, regulatory subunit 159, transient receptor potential channel 5, transient receptor protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRPC5 using anti-TRPC5 antibody (A02701). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC5 antigen affinity purified polyclonal antibody (Catalog # A02701) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPC5 at approximately 111KD. The expected band size for TRPC5 is at 111KD.
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