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ProductsBack/TRPC6 antibody [C3], C-term
Sample(30 μg of whole cell lysate) A:HeLa S3(GTX14654) 7.5% SDS PAGE GTX104730 diluted at 1:3000
Sample(30 μg of whole cell lysate) A:HeLa S3(GTX14654) 7.5% SDS PAGE GTX104730 diluted at 1:3000
Sample(30 μg of whole cell lysate) A:HeLa S3(GTX14654) 7.5% SDS PAGE GTX104730 diluted at 1:3000

TRPC6 antibody [C3], C-term

GTX104730
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetTRPC6
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Overview

  • Supplier
    GeneTex
  • Product Name
    TRPC6 antibody [C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7225
  • Target name
    TRPC6
  • Target description
    transient receptor potential cation channel subfamily C member 6
  • Target synonyms
    FSGS2, TRP6, short transient receptor potential channel 6, TRP-6, focal segmental glomerulosclerosis 2, transient receptor protein 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y210
  • Protein Name
    Short transient receptor potential channel 6
  • Scientific Description
    The protein encoded by this gene forms a receptor-activated calcium channel in the cell membrane. The channel is activated by diacylglycerol and is thought to be under the control of a phosphatidylinositol second messenger system. Activation of this channel occurs independently of protein kinase C and is not triggered by low levels of intracellular calcium. Defects in this gene are a cause of focal segmental glomerulosclerosis 2 (FSGS2). [provided by RefSeq]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRPC6 using anti-TRPC6 antibody (A00625). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat ovary tissue lysate,Lane 2: mouse lung tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPC6 antigen affinity purified polyclonal antibody (Catalog # A00625) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPC6 at approximately 106KD. The expected band size for TRPC6 is at 106KD.
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