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TRPM8 Antibody

LS-C747669
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetTRPM8
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TRPM8 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 5kDa/18kDa/79kDa/127kDa, while the observed MW by Western blot was 122kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 5kDa/18kDa/79kDa/127kDa, while the observed MW by Western blot was 122kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID79054
  • Target name
    TRPM8
  • Target description
    transient receptor potential cation channel subfamily M member 8
  • Target synonyms
    LTRPC6, LTrpC-6, TRPP8, trp-p8, transient receptor potential cation channel subfamily M member 8, TRPM8 cationic channel, transient receptor potential p8, transient receptor potential subfamily M member 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA072616(TRPM8 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)
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Figure 1. Western blot analysis of TRPM8 using anti-TRPM8 antibody (PB9837). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: 22RV1 whole cell lysates, Lane 3: SW620 whole cell lysates, Lane 4: A549 whole cell lysates, Lane 5: A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPM8 antigen affinity purified polyclonal antibody (Catalog # PB9837) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPM8 at approximately 127 kDa. The expected band size for TRPM8 is at 127 kDa.
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