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TRPV1 Rabbit pAb

ORB500680
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetTRPV1
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Overview

  • Supplier
    Biorbyt
  • Product Name
    TRPV1 Rabbit pAb
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 IF, IHC-Fr, IHC-P, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7442
  • Target name
    TRPV1
  • Target description
    transient receptor potential cation channel subfamily V member 1
  • Target synonyms
    VR1, transient receptor potential cation channel subfamily V member 1, OTRPC1, capsaicin receptor, osm-9-like TRP channel 1, transient receptor potential vanilloid 1a, transient receptor potential vanilloid 1b, vanilloid receptor subtype 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NER1
  • Protein Name
    Transient receptor potential cation channel subfamily V member 1
  • Scientific Description
    TRPV1 Rabbit pAb
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRPV1 using anti-TRPV1 antibody (A00128-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRPV1 antigen affinity purified polyclonal antibody (Catalog # A00128-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRPV1 at approximately 95 kDa. The expected band size for TRPV1 is at 95 kDa.
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