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ProductsBack/Tryptase epsilon antibody, N-term
WB analysis of HepG2 cells using GTX44888 PRSS22 antibody at 0.2-1μg/ml.
WB analysis of HepG2 cells using GTX44888 PRSS22 antibody at 0.2-1μg/ml.
WB analysis of HepG2 cells using GTX44888 PRSS22 antibody at 0.2-1μg/ml.

Tryptase epsilon antibody, N-term

GTX44888
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPRSS22
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Overview

  • Supplier
    GeneTex
  • Product Name
    Tryptase epsilon antibody, N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64063
  • Target name
    PRSS22
  • Target description
    serine protease 22
  • Target synonyms
    BSSP-4, SP001LA, hBSSP-4, brain-specific serine protease 4, prosemin, protease, serine 22, protease, serine S1 family member 22, serine protease 26, tryptase epsilon
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9GZN4
  • Protein Name
    Brain-specific serine protease 4
  • Scientific Description
    This gene encodes a member of the trypsin family of serine proteases. The enzyme is expressed in the airways in a developmentally regulated manner. The gene is part of a cluster of serine protease genes on chromosome 16. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRSS22 using anti-PRSS22 antibody (A13704-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRSS22 antigen affinity purified polyclonal antibody (Catalog # A13704-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRSS22 at approximately 42 kDa. The expected band size for PRSS22 is at 33 kDa.
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