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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA849764LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA849764LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA849764LA01HU at dilution of 1:100

TSC1 Antibody

CSB-PA849764LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetTSC1
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Overview

  • Supplier
    Cusabio
  • Product Name
    TSC1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7248
  • Target name
    TSC1
  • Target description
    TSC complex subunit 1
  • Target synonyms
    LAM, TSC, hamartin, tuberous sclerosis 1 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92574
  • Protein Name
    Hamartin
  • Scientific Description
    In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Seems not to be required for TSC2 GAP activity towards RHEB. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Hamartin/TSC1 using anti-Hamartin/TSC1 antibody (A00365-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hamartin/TSC1 antigen affinity purified polyclonal antibody (Catalog # A00365-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hamartin/TSC1 at approximately 160 kDa. The expected band size for Hamartin/TSC1 is at 130 kDa.
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