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TSEN34 Antibody

LS-C817515
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetTSEN34
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TSEN34 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID79042
  • Target name
    TSEN34
  • Target description
    tRNA splicing endonuclease subunit 34
  • Target synonyms
    LENG5, PCH2C, SEN34, SEN34L, tRNA-splicing endonuclease subunit Sen34, CTD-3093M3.1, TSEN34 tRNA splicing endonuclease subunit, leukocyte receptor cluster (LRC) member 5, leukocyte receptor cluster member 5, tRNA-intron endonuclease Sen34
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TSEN34 using anti-TSEN34 antibody (A12386-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TSEN34 antigen affinity purified polyclonal antibody (Catalog # A12386-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TSEN34 at approximately 50 kDa. The expected band size for TSEN34 is at 34 kDa.
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TargetTSEN34
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